FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599706
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68420.2631836061462335No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG57350.22060186805738805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA17700.035.2285432
TCGCCGG17350.034.0512448
GCGGCAC19100.032.66411233
CCCGTCG18350.032.12629344
CCGTCGC18500.031.87391945
ACCCGCG19700.031.70283325
TTCGTTG4600.031.60392631
CGTTGGA4350.031.0747133
CGGCACC20200.030.90400134
TTACTCG2000.029.90776857
CCGCGCG21900.029.02164327
CCCCGAC21700.028.72531920
ACCGACG3800.028.22663130
CCCCCGA22050.027.94511619
GATTTCG3850.027.8319628
TACGGGT4650.027.3589236
AAACCGC3650.027.31840511
TGGCCGT8300.026.67424670
CCCCCGT22200.026.51748742
AGTCCGC23750.026.2038567
Produced by FastQC (version 0.11.5)