FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599706
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68140.26210656128039095No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36720.14124674097763362No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG32210.12389862545995585No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC27690.10651204405421229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG9000.055.3616754
TACGGCC9500.052.8085947
TCTACGG9700.051.7207535
CTACGGC10100.049.6714486
GCGCGGT19800.046.509859
TGGCGCG19700.045.6993915
ACGGCCA12200.041.6847538
GAACGCG12200.041.53929524
GCCATAC12650.039.9302611
CGGCCAT12700.039.7730569
CCCGATC12400.039.05767431
CGCGGTG24450.038.08603710
CGGGTAT3400.037.632461
CTGAACG13600.037.4854922
TGAACGC13850.036.8238123
ATCTCGT13250.036.6445835
GCCCGAT13500.036.62830430
CTACTCG24950.036.60149437
CGCCCGA13650.036.46072429
CGGCATA1900.036.401241
Produced by FastQC (version 0.11.5)