FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576312
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67890.26351622008514497No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG57240.22217805917916772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA17550.038.2888132
TCGCCGG17350.036.48316248
CGTTGGA4850.036.4444333
CCCGTCG17700.035.6671544
GATTTCG4000.035.42086828
GCGGCAC19250.035.10804433
CCGTCGC18000.034.9009745
ACCCGCG19350.034.58738725
CGGCACC20650.032.74839434
ATTTCGT4350.032.58429329
CCGCGCG21600.031.01723527
TTCGTTG5750.030.70756131
CCCCCGA21600.030.43230619
CCCCGAC21700.030.14462520
TCGTTGG6150.029.28704332
CCCCCGT21700.029.05928842
CGTCGCC21800.029.00032446
GTCGCCG21950.028.82151847
GCGCGGG22600.028.76035162
AGTCCGC24200.028.2734417
Produced by FastQC (version 0.11.5)