FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576312
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67200.26083797304053236No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC35090.13620244752964702No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG30620.11885206450150448No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC27390.1063147631187527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC8200.056.5716067
GTCTACG8300.055.8824044
TCTACGG8350.055.143835
CTACGGC8750.053.015686
GCGCGGT20550.047.822569
TGGCGCG20500.047.59932715
ACGGCCA10850.042.7545788
CTACTCG23350.042.21442437
TACTCGG23600.041.6545738
CGCGGTG24550.040.31063510
GAACGCG11650.039.33825724
CGCGTGC25000.039.32928518
ACTCGGG25200.039.16772539
CGGCCAT12150.039.028469
CGGCATA2600.038.617071
GGCGCGT25500.038.53185316
GCGTGCC25800.038.2556919
GCCATAC12150.038.1800211
CGTGCCT26050.038.03465320
AGGATCG23800.037.91073259
Produced by FastQC (version 0.11.5)