FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328998
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA99510.29891877375714854No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC80520.24187458208145513No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC66150.19870844019732065No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT41230.12385108071557868No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38880.11679189954454765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT39350.050.4477969
TGGCGCG42350.047.1119215
CCGAACT7950.044.47076867
CGCGGTG45200.044.071110
GCCGGTT8000.042.21323
CGGTTCA8350.041.2710275
CTACTCG49400.040.647737
TACTCGG49550.040.471238
GTAGTCC49800.040.3158427
TAGTCCC49900.040.17549528
GGGGACT33250.039.5892141
TGTAGTC51000.039.41774726
GGGGTAG14350.039.3478161
CCGGTTC8800.038.7695584
CGGTAAG2800.038.3520551
CCTGTAG52700.038.2553824
GGGGTAC9600.038.2489551
ACTCGGG52950.038.08577739
TCTGGTC28750.037.16107610
CGAACTT9650.036.9918968