FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309966
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG174610.5275280773276826No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC85620.2586733519317117No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC71630.2164070567492234No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG70170.21199613530773426No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45080.1361947524536506No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA41100.12417045975698844No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39210.1184604313156087No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT37050.11193468452546038No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG35010.10577147922365365No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC34720.10489533729349486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA53800.037.37565632
GCGGCAC52950.037.01318733
CGGCACC55000.035.6476934
ACGATCG501.8713085E-434.471286
TCGCCGG54900.034.06200848
CCGTCGC56100.033.5765145
TAGCGTC3650.033.0561511
GCACCCC60500.032.9498436
CGTACGT2950.032.7368219
CCCGTCG58100.032.70234344
TCGCGTG652.1997585E-532.1007541
CCCCCGA64000.031.52654519
CACCCCC63600.031.19639437
GGCACCC64000.030.59269735
TAGTCCG2600.030.4938266
CGATCTA3450.030.3203447
GTTCCGT3100.030.2886141
GATAGGC27300.030.02229940
ACCCGCG67550.029.80638325
CCCCGAC68600.029.3680220