FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325021
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA93170.28020875657627425No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76800.23097598481332898No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC63000.18947248754218393No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT40860.12288644192021644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38820.11675114232361239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT38450.050.5423669
TGGCGCG41150.046.96781515
CCGAACT7850.046.45989267
CGCGGTG43800.044.44747510
GCCGGTT8650.041.8177763
GTACGCA6800.040.71306665
GGGGACT35950.040.5005381
CTACTCG48150.040.3012437
TCTGGTC29950.040.26651410
TACTCGG48450.039.99593438
GTAGTCC48950.039.57009527
ACTCTTC31150.039.1589555
GTTGATC9900.038.98123610
TAGTCCC50650.038.4551928
CGAACTT10050.038.4135168
TGTAGTC50750.038.14704526
GGGACTC36100.037.893322
ACTCGGG51650.037.87191439
CCGGTTC9650.037.127334
CCTGTAG51750.036.99751724