FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239850
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG182490.5632668179082365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98260.3032856459403985No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG61380.18945321542664012No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG60650.1872000246925012No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC53050.16374214855626032No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47370.14621047270706977No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT42350.13071592820655276No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC39270.12120931524607621No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT32450.10015895797644953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA54900.035.83604432
GCGGCAC56300.034.83653633
CGGCACC58900.033.19610634
TCGCCGG56450.032.44456548
CCGTCGC57450.032.0581345
CCCGTCG57900.031.84522244
CCCCCGA63700.031.03121819
ACCCGCG64100.030.69593425
CGCGATT450.00469446230.5718883
GTTCCGT3200.030.1946811
GCACCCC65250.030.04631636
CCCCGAC66700.029.5404920
CACCCCC67600.029.16991637
AGTCCGC68850.029.1236727
CAGTCCG65950.028.6833176
CCGCGCG69350.028.445827
GGCACCC69200.028.37030835
TCCGCCC72700.028.0527139
GTCCGCC71900.028.0317528
CGTCGCC67050.027.3868946