FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250328
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC102070.3140298456032745No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98470.30295404033069895No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA89270.2746492046341169No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC80800.2485902961178072No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT41850.12875623629369098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT47950.053.214399
TGGCGCG49950.050.25831215
CCGAACT8550.048.3720267
CGCGGTG53800.047.5529710
GCCGGTT8950.045.7247283
CGAACTT9650.043.28862868
CTACTCG59300.042.92325637
TACTCGG59600.042.90538838
CGGTTCA9750.041.6189585
GTAGTCC61750.041.55398627
TACGGCC3250.041.2662547
TGTAGTC62500.041.153126
TAGTCCC62550.041.0371528
CGTGCCT61600.040.98908220
ACTCGGG63350.040.9300139
GGGGACT34550.040.536021
CCTGTAG64600.039.567224
TCTACGG3400.039.4456865
AGGATCG63900.039.27851559
CCGGTTC10450.039.160164