FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347665
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG197350.5895153786295821No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101360.3027782051071419No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG65150.19461326028739434No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG64860.19374698483868605No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC55080.1645325921201793No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51580.15407754360128625No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44640.13334667596668126No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC40420.12074087460961595No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT34260.10233998921636424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA59700.035.79796632
GCGGCAC61500.034.65797433
TCGCCGG61200.034.17315348
CGGCACC63450.033.610634
CCGTCGC62650.033.31371745
CCCGTCG63450.033.03409644
ACCCGCG68900.031.61018425
CGTACGT1650.031.30026819
CCCCCGA70150.031.16641219
GCACCCC70250.030.64009336
TAGTCCG4550.030.2402736
CCCCGAC72550.030.05356420
CCGCGCG72500.030.01643227
AGTCCGC73500.029.8586677
CACCCCC73000.029.59886437
CAGTCCG70500.029.2263646
GTCCGCC75950.028.9407758
GGCACCC75400.028.53090535
TCCGCCC78100.028.4964229
CGTCGCC74600.028.08963446