FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307941
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG192170.5809353915320739No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98480.2977078490819516No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG65060.1966782357968295No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63340.19147862673487828No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC54880.16590380541853678No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51130.15456744845207335No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT43820.13246910993878067No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC39930.1207095289789026No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT33450.10112030414085378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA59200.037.0863932
GCGGCAC60950.036.04191633
TTACGCG501.8948411E-434.39803715
CGGCACC64050.034.36524234
TCGCCGG61950.033.29971748
CCCGTCG63150.033.06944744
CCGTCGC62900.032.94956645
GTTCCGT3050.032.755991
GCACCCC69350.031.97352836
TACGCGA652.369811E-531.75299616
CCCCCGA70700.031.173719
CACCCCC72450.030.91172637
ACCCGCG71700.030.56929425
AGTCCGC72650.030.3038487
CAGTCCG70100.030.0812516
CCCCGAC73750.029.85228320
CCGCGCG74300.029.66195727
GGCACCC75100.029.6500535
GTCCGCC75000.029.2625948
TCCGCCC78000.028.798649