FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333934
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG198050.456975117756754No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG96230.22203845282369322No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC79620.18371299609084957No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA64660.1491947039341162No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA62140.14338012530878413No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG57460.13258162214745312No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG43990.10150131497157087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG10350.038.4532670
CGCGGCA61900.036.74477832
GCGGCAC62000.036.58358433
TCGCCGG60100.035.18224748
CCCGTCG60200.035.03217344
CCGTCGC61650.034.1740645
CGGCACC66350.034.09258334
GCACCCC71650.031.8815136
CACCCCC75600.030.2745937
CCCCCGA75850.030.2219519
ACCCGCG76050.030.03960225
GGCACCC77850.029.28909935
CGTACGT2550.028.43080319
AGTCCGC81850.028.2900227
CAGTCCG79200.028.0601586
CCCCGAC84050.027.31896420
TCCGCCC88200.027.3090299
GTTCTCG23250.027.16079513
CGCCCTA26250.027.02111461
GTCCGCC85900.026.9562058