FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333934
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC72400.16705376685477907No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC69110.15946251142726217No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA57040.13161252570989776No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC54260.1251980302422695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT41600.046.203999
TGGCGCG45700.043.11740515
CGGCATA3200.042.219271
CCGAACT7500.040.91081267
CGGGTAG7200.040.415031
GGGGTAG19900.040.3860171
CGCGGTG50550.038.36567310
CTACTCG49600.038.3203137
TACTCGG50500.037.37436738
GTAGTCC52450.036.85341327
GGTACGA2450.036.622673
CGGCGTA2100.036.2910461
GGGGTAC13500.036.1810761
TAGTCCC53650.035.77874428
CGGCAAA8700.035.437811
TGTAGTC54550.035.42360326
ACTCGGG57400.034.04271739
CGTGCCT57150.033.89653420
CGGTTCA9100.033.7490275
GCCGGTT9350.033.58713
Produced by FastQC (version 0.11.5)