FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441714
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG215400.48494792775941903No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG102580.23094688221709006No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC82400.18551397050778146No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA69070.1555030332885008No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA61390.13821241079457164No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG61040.13742442669654104No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG46370.10439663607337167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAC68700.036.54157333
CGCGGCA69100.036.01675832
TCGCCGG66600.035.31815348
CCCGTCG66600.035.16996844
CCGTCGC66800.035.0889945
CGGCACC72400.034.20977834
AGGACCG10050.032.51000670
GCACCCC76850.032.43557736
CACCCCC80150.031.11455537
CGTTGCG2000.031.1123071
CCCCCGA82250.030.67644119
ACCCGCG84450.029.74397325
GGCACCC83900.029.65497635
AGTCCGC88150.028.6539847
CAGTCCG85100.028.2617786
TCCGCCC93650.027.8554559
GTCCGCC92100.027.4250668
TTATCGG2900.027.366937
GTTCCGT3800.027.2914961
CGCCCTA25700.027.19986761