FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4048994
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG259540.6409987270912232No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN227170.5610529430273298No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG91300.22548810889816087No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG66600.1644853017811338No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA47010.11610291346443093No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC45830.11318860931875917No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG41280.10195125011299103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA73300.038.40078732
GCGGCAC75150.037.58807433
CGGCACC78200.036.15941634
TCGCCGG75050.035.590548
CCCGTCG76700.034.9735444
CCGTCGC76250.034.9002645
CCCCCGA84850.033.29395319
GCACCCC86850.032.7000536
ACCCGCG89350.031.72271525
CACCCCC89750.031.71255537
GGCACCC92800.030.68916335
CCCCCGT88900.030.06606542
CCCCGAC95050.029.70774520
TAGTCCG4450.029.1003426
AGTCCGC97550.028.7855457
AGGAGGG136450.028.4879570
CCGCGCG100200.028.30228627
CAGTCCG95350.028.2345526
CGTCGCC94950.028.09595946
TCCGCCC104300.027.8030729