FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4093391
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG276080.6744530390573488No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229900.5616370388267331No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG98350.2402653447960383No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG71640.17501382105936128No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA49160.12009602796312396No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC47190.11528339218022418No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG43020.1050962392793652No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC42940.10490080229325759No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG42180.10304415092523533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA81050.036.76104732
GCGGCAC83550.035.52673333
CGGCACC85900.034.66765234
TAGTCCG5150.034.405646
TCGCCGG82000.034.40275248
CCGTCGC82750.034.1276645
CCCGTCG84000.033.8283844
CCCCCGA92250.032.43650419
GCACCCC93950.031.94578236
CACCCCC97200.031.0268537
GGCACCC98250.030.54743235
ACCCGCG98400.030.51313225
AGGACCG8950.029.17054470
CCCCGAC104050.028.9102920
CCCCCGT98150.028.81451442
AGGAGGG140650.028.64391570
AGTCCGC107150.028.2714757
CAGTCCG103050.027.6764876
CCGCGCG108750.027.56253227
CGTCGCC103950.027.40234646