FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205585
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG280980.6681115706851721No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN235280.5594465454865375No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG100000.23777904857469295No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG72230.17174780678550072No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA49650.11805729761733505No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC46920.11156592959124592No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG45070.10716701719261411No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG42480.10100853983452955No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC42290.10055675964223765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA82250.037.61749632
GCGGCAC84250.036.8805233
CGGCACC87450.035.57280334
TCGCCGG83900.034.9784148
CCCGTCG84800.034.50798844
CCGTCGC85900.033.9877645
CCCCCGA94500.032.89494319
GCACCCC96550.032.54277436
ACCCGCG98400.031.6977525
GGCACCC100100.031.32048835
CACCCCC101250.031.06237437
CCCCGAC104300.029.78888120
CCCCCGT99450.029.32831442
AGGAGGG145700.028.51204970
CCGCGCG111850.028.03142427
AGTCCGC112650.027.9289347
CAGTCCG108400.027.5774566
CGTCGCC106800.027.47406246
CGCCCTA16900.027.30024561
TCCGCCC119500.027.2122469