FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4162567
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG274070.6584158285019797No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN231580.5563393934560092No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG95600.22966597294409916No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG71200.17104829784121192No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA47940.11516931739477107No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC46980.11286304821039517No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG44960.10801027346827091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA84300.038.22084432
GCGGCAC86250.037.4370933
CGGCACC88300.036.4047534
TCGCCGG84650.035.25614548
CCCGTCG84750.035.11285844
CCGTCGC85250.034.99261545
GCACCCC95050.033.921636
CCCCCGA95050.033.7749619
CACCCCC99350.032.6648437
GGCACCC101350.031.81202735
ACCCGCG102100.031.3525125
TAGTCCG5950.030.9305236
CCCCCGT99350.030.02254942
CCCCGAC107800.029.76982320
AGGAGGG145900.029.72995270
AGTCCGC112550.028.4335167
CGTCGCC105500.028.31432746
CCGCGCG114850.027.91572827
CAGTCCG108400.027.7299946
GTCCGCC116900.027.3463158