FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4019980
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG207080.5151269409300544No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG120160.2989069597361181No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC110140.27398146259434125No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG100470.2499266165503311No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC75600.1880606371175976No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT65420.16273712804541315No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC57130.14211513490116867No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54180.1347767899342783No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA53470.1330106119930945No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT53440.13293598475614304No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG48810.12141851451997275No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA47890.11912994592012895No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC47350.11778665565500326No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA42020.10452788322329962No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC41870.10415474703854247No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT41830.10405524405594058No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT41220.10253782357126154No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG41170.10241344484300918No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG40310.10027413071706824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG702.1465894E-839.5214736
CGCGGCA64800.036.00644332
GCGGCAC65900.035.46467233
CCGTCGC63450.034.41891545
CGGCACC67750.034.39991434
CCCGTCG64050.034.1838244
TCGCCGG64650.033.7689448
GCACCCC71500.032.71397436
GTTCCGT3500.032.6662181
GTTATCG2500.031.8147856
ACCGTTA1101.0131771E-931.4963571
CGTACGT2750.031.44732319
AGGACCG9300.031.3676270
CCCCCGA75550.031.36407519
CACCCCC75850.030.8482337
TAGTCCG4750.030.577146
GGCACCC77400.030.25414735
TATCGGA2700.029.4581388
ACCCGCG80550.029.14163225
TACGATC953.1434865E-729.1192725
Produced by FastQC (version 0.11.5)