FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4019980
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA173180.43079816317494124No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC96170.23923004592062647No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC82900.206219931442445No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT42680.10616968243623101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT48500.049.610699
TGGCGCG52000.046.802215
GGGGACT49650.046.560561
ACTCTTC47150.046.559755
TCTGGTC46700.045.74876410
CGGCAAT3500.045.6197661
GGACTCT50500.044.632153
GTCTACG5050.044.4952354
CGCGGTG56650.042.71750610
CTGGTCC50200.042.49024211
GGGACTC52450.042.3796162
CGGGACT9750.041.2968181
TTCTGGT52400.041.1681259
TACGGCC5400.040.973667
CGGCATA2550.040.83611
TCTACGG5600.040.7450185
GGGGTAG18150.040.7348561
CTACTCG60100.040.60168537
GTAGTCC60750.040.0690927
TACTCGG60950.040.05098338