FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302877
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC202580.6133440633726294No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC167520.5071941825263248No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG107350.32501967224332No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT84610.25617060520267637No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70900.2146613391900455No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC44900.13594208927550133No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC39440.11941104679344704No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC37420.11329516660777861No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC33710.1020625351776648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG23150.060.2973485
TACGGCC23450.059.2343447
GTCTACG23750.058.7740484
CTACGGC24300.057.867196
GCGCGGT93750.054.912459
GCCGGTT12800.053.8535773
TGGCGCG96700.053.1260115
ACGGCCA26250.052.916018
CGCGGTG97750.052.7355710
CCGAACT13350.052.6644167
CGGCCAT26950.051.5415739
CCGGTTC13650.050.754384
GGTCTAC22750.050.6508033
GCCATAC27650.049.85854711
CCTGGTT42400.049.46488670
CGGTTCA14200.049.2715955
CGGCATA2600.049.051231
CGAACTT14950.048.53936868
GGGTCTA22650.046.7863042
GAACGCG29500.046.63516624