FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286950
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG181050.5508145849495734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70110.21329804225802035No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG55990.17034028506670318No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46970.14289843167678243No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG38330.11661266523676965No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG34150.103895708787782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52500.037.199532
GCGGCAC53450.036.42743333
TCGCCGG52700.035.61186248
CGGCACC55450.035.25776734
CCGTCGC54700.034.3491345
CCCGTCG55800.033.89356644
TGGCCGT14400.033.45390770
GTCGGTT2900.033.160334
CCCCCGA59750.033.02353719
TAGTCCG3750.032.9708446
CCCCCGT58850.032.09084342
CCCCGAC61800.031.99543220
AGTCCGC63150.031.5981487
ACCCGCG62250.031.53798725
GCACCCC62950.031.48198536
AGGAGGG88900.030.99818270
CAGTCCG61300.030.5908156
CCGCGCG65600.030.1637327
GTCCGCC66750.029.945438
CACCCCC66700.029.7914437