FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639022
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC279220.7672940696703674No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC242650.6668000358338037No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG177780.4885378544015398No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT137930.3790304098189019No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA117540.3229988716748621No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC62100.17065024613756113No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC56080.15410733982921784No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC55300.15196390678594413No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC49860.13701483530465053No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG48470.13319512770189354No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC47260.12987005849373817No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43710.12011469015576162No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC38960.10706173252044092No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38640.1061823753744825No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCGTC38410.10555033742582484No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38370.10544041778258004No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT37200.10222526821766946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA353.5197445E-970.48173560
TCTACGG35750.062.7684175
GTCTACG36100.061.968894
TACGGCC36500.061.384227
CTACGGC36750.061.0604326
ACGGCCA39350.057.288748
CGGCCAT39650.056.6814089
GCCATAC39450.056.5233411
CGGTTCA20900.056.2398725
GGGTCTA29950.055.4705622
GCGCGGT124650.055.0020339
CCTGGTT58000.054.85231470
GCCGGTT21400.054.7617723
CCGAACT21500.054.7279367
CATACCA41500.054.15241213
CCATACC41450.053.9682712
CGCGGTG127650.053.817410
GCGTCTA7250.053.7220762
CCGGTTC21950.053.3925444
GGTCTAC35300.051.457453
Produced by FastQC (version 0.11.5)