FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618086
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC275740.7621156600478817No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC243650.6734223564613997No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG173000.4781533661720589No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT135440.37434157175921196No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA116590.3222422020924876No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC61830.17089146029143587No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC54190.14977532319574494No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC53860.148863238740041No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG49690.1373378078906914No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48270.1334130808388745No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC45610.12606112734744282No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG42450.1173272277110052No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38900.10751541008146297No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38350.10599526932195641No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCGTC36940.10209818119303964No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC36520.10093734643123464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG33050.062.7970964
TCTACGG33600.061.9743775
TACGGCC34250.060.595237
CTACGGC35250.058.877036
ACGGCCA37800.055.360428
GCGCGGT130150.055.016749
GCCATAC38150.054.8525311
CGGCCAT38650.054.0537269
GCCGGTT18500.054.043173
CCGAACT19250.053.7033167
GGGTCTA27800.053.3487742
CGCGGTG134500.053.31428510
CGGTTCA19050.053.025795
CGCGTAA407.1054274E-752.9013461
CCTGGTT56050.052.83484370
GCGTCTA7650.052.7503362
TGGCGCG138600.051.66254415
CCATACC40500.051.41436412
CCGGTTC19650.051.4066854
GGCCATA41050.051.1454110
Produced by FastQC (version 0.11.5)