FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633286
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC281820.7756614810945244No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC243010.6688435757603447No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG177130.4875201126473391No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT136310.3751700251507864No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA114520.3151967667835673No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC62840.1729563816335956No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54540.1501120473312588No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC54390.14969919791615635No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG50030.1376990415838445No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48380.13315769801771732No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC46300.12743285279496303No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43980.12104744850804479No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC37910.10434080884356475No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37540.10332244695297865No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCGTC37070.1020288521189909No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36860.10145086293784744No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT36720.10106553681708515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG35250.062.2075084
TCTACGG35400.062.0413135
TACGGCC36200.060.764647
CTACGGC36550.060.1811076
CCGAACT19750.057.4110467
CGGCCAT38950.056.5629739
ACGGCCA39400.056.1794668
GCGCGGT125000.055.853949
GGGTCTA28800.055.7899782
GCCATAC39350.055.46228811
CGGTTCA20500.054.8287775
CGCGGTG129700.054.04260310
GCCGGTT20900.053.7809033
CCTGGTT58000.053.6791170
CCGGTTC21050.053.559994
CCATACC41150.053.03623612
TGGCGCG132300.053.0319315
GGCCATA42050.052.14707610
CGAACTT22700.051.74637268
GAACGCG42850.050.9316524
Produced by FastQC (version 0.11.5)