FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631248
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC265910.7322826752675664No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC238860.6577903795058889No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG170810.4703892435878794No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT131870.3631533841808656No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA111740.30771789753825685No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC60060.16539768145827552No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC53570.14752503822377322No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC53450.14719457332575467No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG50070.13788647869823267No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC47030.12951470128176318No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44940.1237591043079404No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43750.12048199406925664No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37180.10238904090274198No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36820.10139764620868638No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC36610.10081933263715395No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCGTC36360.10013086409961533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG36100.064.1811755
GTCTACG37150.062.5510674
TACGGCC37600.061.7116057
CTACGGC37700.061.4573146
ACGGCCA39500.058.8304868
CGGCCAT41150.056.471559
GCCGGTT17400.056.471493
CCTGGTT58000.056.09379670
GCCATAC41200.055.81645611
GCGCGGT122650.055.54689
CGGTTCA17900.055.4734345
CCATACC42400.054.23375712
CGCGGTG126950.053.77323510
CCGAACT18750.053.76488567
GGGTCTA31450.053.6299742
CATACCA43200.053.30922713
GCGTCTA7850.052.7267462
CGTCTAC8900.051.9096873
CCGGTTC19150.051.8510254
TGGCGCG131250.051.82406215
Produced by FastQC (version 0.11.5)