FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3798834
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG234890.6183213059586178No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG94240.24807612019898737No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93410.24589123925920428No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG72970.19208525563370232No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG62270.16391871821722137No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC50030.13169830532210675No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT47560.12519631023624617No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC46120.1214056734250562No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA44310.11664105354432439No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC41400.10898080832171134No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT40430.106427393247507No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG38190.10053084709676706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA69600.038.6293832
GCGGCAC71650.037.54058533
CGGCACC72600.036.9676134
CCGTCGC70700.035.86992645
TCGCCGG70950.035.5235648
CCCGTCG71500.035.49317644
GCACCCC78200.034.48678636
CTACCGG1505.5479177E-1033.3206470
CACCCCC81750.033.13331237
GGCACCC83500.032.1996835
CCCCCGA83550.032.09847619
ACCCGCG84200.031.88006225
TTATCGG1800.030.6136157
CGTAGCG5400.030.6124029
CCCCGAC89050.030.12671920
GTAGCGT5650.029.2578710
CCCCCGT87000.029.13621342
AGTCCGC95950.028.2485967
TAGCGTC5900.028.01813111
CGTCGCC90900.027.96037546