FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768236
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG250670.6652184205023252No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG98410.2611566791464229No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90040.2389446945467322No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG74120.1966968098601043No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG66710.1770324363972957No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC50270.13340459567819002No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT48660.12913203950071067No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45180.11989694912951311No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC43940.11660628474437376No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG41120.10912267702978265No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC40940.10864499994161723No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39860.1057789374126249No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC38380.10185137024326502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA74200.037.03278432
GCGGCAC77550.035.45298433
CGGCACC79450.034.5814334
TCGCCGG76850.034.0826548
CCCGTCG77350.033.9523344
CCGTCGC78100.033.42642645
GCACCCC86150.032.09005436
CCCCCGA87550.031.93451119
ACCCGCG89350.031.12186425
CACCCCC90400.030.83011237
GGCACCC89950.030.63948235
CCCCGAC93450.029.74059720
CCCCCGT91200.028.68182842
GTTATCG2050.028.5507976
AGTCCGC101950.027.489097
CCGCGCG101950.027.26164427
CAGTCCG100500.026.9264856
CGTCGCC97850.026.76759546
CCCGACC104200.026.71327221
GTCCGCC105050.026.6778958