FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768236
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89060.23634400817783177No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA77200.2048703955909343No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC75310.1998547861651977No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC70150.1861613763044565No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC58620.1555635050458623No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40210.10670775397294649No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38130.10118792984303532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT41350.047.6952749
TGGCGCG43900.044.68901415
CGCGGTG47300.041.9821510
TGGTCCG12300.041.41013312
TACTCGG49700.039.86469338
GGGGACT41100.039.723551
CCGAACT6750.039.5998467
TCTGGTC37400.039.47623410
CTACTCG49900.039.40722337
ACTCTTC37800.038.8805435
CGGGTAG5300.038.4242061
GTAGTCC52600.038.10188327
CGGCTAT2650.037.772951
ACTCGGG54700.037.57994539
TAGTCCC53350.037.51951228
GGACTCT41100.037.4336283
CGTGCCT53750.036.7478220
TGTAGTC54900.036.5539226
CTGGTCC40900.036.01392711
TTCTGGT41200.035.9225779