FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759570
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG250230.6655814361748817No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG98230.26127988041185557No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84650.22515872825881683No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG74930.19930470771923384No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG63250.1682373250132329No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT48210.128232750021944No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC45840.12192883760642839No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA43570.11589091305654636No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC43440.11554512883122273No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC41190.1095604018544674No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG40340.10729950499658206No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT40090.10663453533249813No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39780.10580997294903406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG853.0049705E-936.48963
CGCGGCA72350.035.58961532
GCGGCAC74950.034.4618233
CGTACGT2250.033.72220219
CGGCACC77450.033.3203134
TCGCCGG74400.032.0321948
CCCGTCG75950.031.48163244
CCGTCGC76150.031.23060845
GCACCCC82650.031.21128736
CGATCTA2650.030.30271347
CCCCCGA85350.030.22552519
ACCCGCG86200.030.11542325
CACCCCC86250.029.92101737
GGCACCC87700.029.39786735
CCCCGAC90300.028.54263920
TACGTAG2750.027.61042221
AGGACCG6800.027.33038570
CCCCCGT89750.026.57063542
AGTCCGC99650.026.2425357
CCGCGCG99250.026.14111527
Produced by FastQC (version 0.11.5)