FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3984653
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG427361.072514971818123No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC197000.4943968772186687No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC180240.4523354982228064No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA152940.383822631481336No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG115650.2902385728443606No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT93970.23582982006212336No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80500.2020251198786946No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA74710.18749436902033878No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC60690.15230937298680713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54680.13722650378841017No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG50910.1277652031431595No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC48290.12118997563903305No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC46450.11657225861323434No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC42000.10540441037149283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG85900.064.896784
TCTACGG86250.064.633435
CTACGGC87450.063.5501336
TACGGCC86800.063.509217
GGGTCTA64500.061.7780572
ACGGCCA89700.061.572388
CGGCCAT91300.060.3059969
GCCATAC91350.059.93760311
CCTGGTT131750.059.32270
CCATACC92650.058.9861212
CATACCA94050.058.28994813
GGCCATA94700.058.17784510
CGTCTAC19050.058.0597343
GCGTCTA16450.058.051692
TACCACC99300.055.62325315
CGGTCTA10150.055.502662
ATACCAC101100.054.73485614
GCGCGGT94550.054.6729779
CCGAACT13750.053.99746367
ACCACCC103900.053.22741716
Produced by FastQC (version 0.11.5)