FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978612
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG214270.5385546517227616No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC166700.4189903413552264No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG154240.38767288692639545No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC143360.3603266666867742No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG95000.2387767392246341No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT92700.2329958286960377No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT82260.20675552177493056No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA76490.19225297666623434No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT73510.18476292737266162No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG67290.16912933455184873No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT63980.16080985026939043No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG61680.15502893974079401No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG59870.15047961449872468No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG57290.14399494094925566No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG57020.14331631232198566No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC56360.1416574423442145No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55820.14030018508967448No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG55240.1388423902607241No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT52840.13281013579610176No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA52530.13203096959442137No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA51140.12853728888366092No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG50140.12602384952340162No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC47070.11830759068740557No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT46270.11629683919919812No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG45850.11524119466788921No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG45080.11330584636048953No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT43960.11049079427699912No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTT43890.11031485352178097No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG43120.10837950521438129No Hit
TGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG42220.10611740979014793No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC41640.10465961496119752No Hit
GGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTG39820.10008515532552559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT34450.057.6967370
AGGACCG8900.038.81584570
GTTCCGT4400.038.321821
ATGGCCG41850.038.10267369
ACGATCG1100.037.496756
CGCGGCA63000.036.8020832
GCGGCAC64050.036.27474633
CGGCACC66650.034.97936634
TCAGGAT25200.034.7686270
CCGTCGC64600.033.57590545
TCGCCGG65300.033.19949748
CCCGTCG66300.033.00859544
GGCACCC73200.032.00776735
CACCCCC74150.031.58543637
GCACCCC75250.031.47288336
TATGGCC47600.031.4696868
GTGCCGT5600.031.3389821
CCCCCGA74850.031.2928219
TTCCGTT6150.030.751592
GTATGGC48050.030.63983267
Produced by FastQC (version 0.11.5)