FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978612
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG425191.0686892815886546No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC206690.5195027813719961No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC186280.4682034840291036No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA159580.4010946531101801No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG114900.28879418249379435No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT96420.24234582311620234No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80960.20348805060659345No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA76650.19265512696387585No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC63410.15937718983404262No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55860.14040072266408485No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG51540.12954266462776465No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC51000.12818540737322462No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC47170.11855893462343148No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC41630.10463448056759495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG81050.064.622374
TCTACGG82250.063.7213335
TACGGCC82550.063.489767
CTACGGC82950.063.1835986
CGTCTAC17900.061.4395263
ACGGCCA85650.061.1918228
GGGTCTA60700.060.4812772
GCGTCTA15850.060.0743262
CGGCCAT87750.059.6882369
CCTGGTT124400.058.8738970
GCCATAC88700.058.81722611
GGCCATA90800.057.75899510
CCATACC90450.057.7552412
CATACCA91750.056.93691313
CCGAACT13800.055.27420467
TACCACC94350.055.04006615
GCGCGGT98650.054.765419
CGGTCTA10500.053.0351872
ATACCAC99350.052.7889514
CTGAACG99550.052.1395722