FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028153
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG425941.0574077002536895No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC201250.4996086295629784No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC182170.4522420076893802No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA159970.397129900477961No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG115670.2871539387903091No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT93120.23117294700573687No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80770.2005137342101951No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA78500.19487839712145988No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC61610.1529485101484477No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56920.1413054568681974No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG49840.12372916321698804No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC49770.12355538630235743No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC44970.11163925501340193No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC43270.10741895851523017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG81800.064.8007354
TCTACGG82100.064.524495
TACGGCC83750.063.1719447
CTACGGC84300.062.7190066
GCGTCTA15950.060.9932
ACGGCCA87150.060.7468578
CGGCCAT87950.060.19289
GGGTCTA62600.059.8558272
GCCATAC87950.059.4501611
CCTGGTT124550.058.33956570
CGTCTAC18950.058.230443
CCATACC89900.058.19670512
CATACCA91150.057.43561613
GGCCATA92000.057.31881310
CCGAACT13800.055.7296467
TACCACC95600.054.83537715
GCGCGGT94100.054.3591889
ATACCAC97800.053.6370114
GCCGGTT14100.053.3923723
CGGTCTA10200.053.248962