FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4021093
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG261780.6510170244756837No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG112400.27952598957547115No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84850.2110122794971417No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC74410.1850491893621958No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG68630.170674988118902No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG67650.1682378398112155No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC60730.151028588495715No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT58050.1443637339400009No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA52350.13018848357896723No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT46990.11685877446753906No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG46710.11616244637962864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA73350.037.4491432
GCGGCAC76400.036.15723833
CGGCACC78600.035.16726334
TCGCCGG74250.035.08543448
CCCGTCG76000.034.36891644
CCGTCGC77800.033.41807645
TGGCCGT15350.032.3816770
GCACCCC86550.032.28715536
CCCCCGA85550.032.12738419
ACCCGCG85700.031.86332525
CACCCCC87350.031.61112437
CCCCGAC88200.031.172620
CCGCGCG92050.029.61393527
GGCACCC93650.029.60427935
CGTCGCC90800.028.72892846
GCGCGGG92150.028.64481762
CCCCCGT90900.028.54831142
GTTCCGT4050.028.1455331
CCCGCGC97750.027.94414726
AGTCCGC101350.027.371637