FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4021093
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC150820.3750721507808947No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC120540.2997692418454385No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA114720.2852955651610147No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG105350.2619934430762979No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84240.20949527901990825No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT60180.14966080118017663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23700.060.3976974
TCTACGG23900.060.036255
TACGGCC24100.059.254697
CTACGGC25350.056.4664886
GCGCGGT76650.052.2106639
ACGGCCA28550.050.5009548
GCCATAC28700.049.38849611
TGGCGCG81100.049.29541815
CCGAACT11050.048.67243667
CGGCCAT29700.048.197949
GGGTCTA23200.047.0108342
CATACCA30950.045.91378813
GGCCATA31050.045.88073710
CGCGGTG87750.045.76309210
CGGTTCA11500.045.4800725
CCATACC31100.045.47107312
CTGAACG31200.045.18528422
GAACGCG31000.044.9522824
TGAACGC31800.044.12972323
CCCGATC31800.044.0862831
Produced by FastQC (version 0.11.5)