FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4003426
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC148220.37023289552498284No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC121480.3034401035513083No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA112070.2799352354708192No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG103960.25967758614746467No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81770.20425005982376096No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT59700.14912227676994655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG22600.060.2925644
TACGGCC22800.059.612027
TCTACGG23150.058.860135
CTACGGC24500.055.4763686
GCGCGGT72750.052.640859
ACGGCCA27300.050.2887658
GCCATAC27500.049.5476711
TGGCGCG78800.048.77453215
CGGCCAT28250.048.3540429
CCGAACT10600.047.3519167
CGCGGTG82800.046.41769410
CGGTTCA11300.045.6761865
GCGTCTA5850.045.3085752
CCATACC30100.045.1534912
GCCGGTT11450.045.0778123
TGAACGC30250.044.359823
CATACCA31100.043.9234313
GAACGCG30550.043.82831224
CTGAACG30750.043.51103622
GGGTCTA23150.043.418762
Produced by FastQC (version 0.11.5)