FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005170
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC148170.3699468437045119No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124480.3107982932060312No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA109520.27344657030787706No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG103670.25884044872002937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82290.20545944366905772No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT59380.14825837604895672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG22500.058.302015
GTCTACG22650.057.7638974
TACGGCC22900.057.2836347
CCGAACT10250.053.12545467
CTACGGC24700.053.107136
GCGCGGT75000.051.2782259
GCCATAC25650.051.27570311
CGGCCAT26900.048.765629
ACGGCCA27100.048.5327768
TGGCGCG80150.047.7238315
GCCGGTT11400.047.7192153
CGAACTT11700.047.3871668
CCATACC28400.046.31062712
CATACCA29050.045.39350513
CGCGGTG85400.045.1948310
CGGTTCA12150.044.7735825
GGGTCTA22700.044.744252
GAACGCG29050.044.20479624
CTGAACG29400.043.53328322
TACCACC30750.043.21822715
Produced by FastQC (version 0.11.5)