FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173367
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG115550.3641242881771947No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG55590.17517671293613377No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG52630.1658490808028192No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC48890.15406349155329338No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA45880.14457829806637557No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA40710.12828645410379574No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC36750.11580759489841547No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCC35120.11067109477094833No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC34090.10742533088672064No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG31910.10055565586961734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT13450.040.17309670
CGTACGT1600.034.60321819
GTCCGTT2050.033.750722
CGCGGCA35750.032.97071532
TCGCCGG34400.032.71578248
CCCGTCG34850.032.64142244
CCGTCGC34900.032.59930845
GCGGCAC36950.031.81215733
ATGGCCG14250.031.03612769
TACGCGT450.00456245230.7506562
CGGCACC38450.030.66468434
GTTCTCG17300.030.39180413
TCGCCGT2450.029.7603471
CACCCCC41650.028.66348537
GCACCCC41950.028.4499536
CGCCCTA18800.027.67203361
GTACGGC9900.027.60265714
CCGATCC7450.027.533570
CCCCCGA43700.027.39751219
GTTCCGT2800.027.280321