FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005318393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756224
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN352120.937430781550834No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC126010.335469876130923No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG118920.3165945374929717No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC108500.2888539128656864No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA103740.27618161217222403No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA65830.1752557887921487No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51950.1383037859296996No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC50980.13572140532619992No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT46170.12291599223049529No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41120.10947163960402788No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG40440.10766131093353326No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37680.10031350632976097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)