FastQCFastQC Report
Wed 8 May 2024
EGAF00005318395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864417
Total Bases283 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN361680.9359238405172113No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT260490.674073217253728No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG122560.3171500384145914No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG111840.28940976090313236No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA65470.16941753439134544No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT57410.14856057200866263No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA54710.14157374838170933No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC51550.13339657702571953No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT48220.12477949455247714No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT47290.12237292196985987No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTG44460.11504969572383106No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCC43890.11357469962480758No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC41230.10669138449603136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)