FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8895
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN680.7644744238336143No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG370.41596402473299604No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC180.2023608768971332No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC180.2023608768971332No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA160.17987633501967398No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG120.13490725126475547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTCT150.002474369767.5795443
CTCTTCT150.002474369767.5795446
GGGACTC150.002474369767.5795442
TCTTCTG150.002474369767.5795447
GGGGACT309.181549E-656.6380961
GACTCTT200.007730408650.684664
GGGGGGC756.1008217E-527.1862851
Produced by FastQC (version 0.11.5)