FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092119
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG164480.5319329560084848No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC133980.4332950963400827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN132950.4299640473086579No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC110650.3578452187642196No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG78470.2537741917435907No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT58120.18796171816155846No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG56300.18207578686331283No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG48890.15811163800616987No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46220.14947678274995238No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG45980.14870061598534856No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44100.14262064299595198No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG39510.1277764536229039No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39070.12635348122113024No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA38360.12405732120917727No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC36840.1191415983666864No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA35600.11513140341623335No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG32420.1048471937852327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA209.2654554E-567.835262
TGGCCGT27050.050.29495270
TTACGCG350.001467938338.75167515
TCAGGAT22950.037.6417470
CGCGGCA51750.036.55415332
GCGGCAC52550.035.97072233
CGGCACC52600.035.90659334
AGGACCG10250.035.83194470
CGTACGT1350.035.24080719
ATGGCCG31200.034.28862869
TACGTAG1500.034.02982721
TACGCGA400.002827127733.90771516
CCGTCGC52500.032.92006745
GGCACCC57850.032.6911635
CCCGTCG53850.032.43351444
TCGCCGG53800.032.1539948
TTATCGG1800.032.0239567
CCTATCG851.4044781E-731.9224762
CCCCCGT54150.031.83490442
CTATCGA751.9117142E-631.6482323