FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181989
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG170400.5355141076854759No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC140690.4421448345673099No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136990.4305168873933883No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC114520.3599006784750042No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG81460.25600339913180087No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT60260.18937840451365484No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57220.1798246317004867No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG51580.16209986898131956No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47750.15006337231209788No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG47060.14789491729858276No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44140.13871826709646073No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG40570.12749886941783894No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA39660.12463902295073931No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39490.12410476591842398No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC37800.11879362247952459No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA36710.11536809209585577No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG34220.107542797916649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT28150.051.11655470
CGCGGCA53700.037.7273732
TACGCGT451.1074587E-437.6870572
GCGGCAC54250.037.1909833
CGGCACC54700.036.9205134
TCAGGAT21900.036.8993370
AGGACCG9550.036.5765170
ATGGCCG32450.035.4225569
CCGTCGC55900.034.09002345
CCCGTCG56300.033.79778344
CCCCCGT56250.033.60814342
TCGCCGG56750.033.5682548
GGCACCC60600.033.47886735
CCCCCGA61250.033.01542719
GCACCCC62100.032.87330236
ACCCGCG63250.032.24311425
CACCCCC65350.031.2806737
CCCCGAC65700.030.8529120
TATCGGA2250.030.1472668
CCCCGTC63550.029.95316143