FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181989
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG294370.9251131917803613No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136520.4290398238334576No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC128200.4028926561342606No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC118920.37372850754669484No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA116250.3653375294509189No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80960.25443205491910875No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT56980.1790703864783945No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG56140.17643052820107172No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC50430.15848577729212765No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA36670.11524238455884041No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG36550.11486526194779428No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC33270.10455724391253395No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC32380.10176025121394197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG58200.063.6137585
GTCTACG58250.063.5591554
TACGGCC58000.063.2605447
CTACGGC58650.062.902386
ACGGCCA59900.061.1407178
GGGTCTA44250.060.682482
CCTGGTT89600.059.49468270
CGGCCAT61250.059.4055869
GCCATAC62050.058.4210811
GGCCATA63650.057.3254610
CGTCTAC13750.056.7122043
CCATACC64100.056.3939912
CATACCA64700.056.131313
GCGTCTA12350.052.983632
TACCACC68500.052.91008815
CTGAACG69850.052.01862722
GCGCGGT65800.051.5871859
ACCACCC71500.051.07346716
CCGAACT10550.050.56984367
CGCGGTG68100.049.99426710