FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150419
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG289240.9181001003358601No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131890.41864272657065615No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC125230.3975026813893644No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC119310.37871153011710507No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA114110.36220578913471513No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG82190.26088593295050594No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT57060.1811187654721483No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54760.1738181492683989No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC50230.15943910952797072No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA37100.11776211354743607No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34720.11020756286703451No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC32700.10379571733156764No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC32190.10217688504291017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG60550.063.6293954
TCTACGG61100.063.113145
TACGGCC61100.063.058647
CTACGGC61850.062.2939726
ACGGCCA63500.060.568498
CCTGGTT92450.060.4175970
CGGCCAT65000.059.4297569
GGGTCTA46350.058.6106262
GCCATAC66300.057.75292211
GGCCATA67300.057.0459710
GCGTCTA11500.056.918452
CCATACC68100.056.1757212
CATACCA68500.055.84679813
CGGTCTA8450.054.585312
CCGAACT9350.054.30259367
CGTCTAC13750.054.017573
TACCACC71000.053.92898615
CTGAACG73550.051.7210722
ACCACCC74350.051.6359416
GCGCGGT65950.051.1684079
Produced by FastQC (version 0.11.5)