FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223069
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG129820.40278380636592015No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91910.28516299216678265No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61280.19012934566402392No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG39270.12184039497758192No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34420.10679262528974713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19900.039.19981870
GTTCCGT3700.037.982491
CGCGGCA39600.036.50413532
GCGGCAC43850.035.43017233
CGGCACC44850.034.66472234
TCGCCGG43100.034.28288748
CCGTCGC43900.033.7886145
CCCGTCG45300.033.01582344
ATGGCCG19150.032.4413769
AGGACCG8600.031.62794570
CCCCCGA50000.030.87534519
GACGTAT6500.030.57204844
GCACCCC51900.030.26648136
CGTACGT1600.029.95113819
GGCACCC52950.029.64714835
ACGTATC6850.029.55175645
CGTATCA6900.029.37215446
ACCCGCG53200.029.1684425
GTCGGTT3300.028.9978834
AGGAGGG76950.028.67829770
Produced by FastQC (version 0.11.5)