FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212893
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG154320.48031478172475706No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC134220.4177543416478544No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC130340.4056779979912185No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN92540.2880270211301777No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT68710.2138571063524369No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC57760.17977567257919888No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39900.12418714224220974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG30950.062.377084
TACGGCC31750.060.805377
TCTACGG32250.060.180515
CTACGGC32500.059.507316
GGTCTAC28500.054.792633
ACGGCCA35550.054.4980138
CGGCCAT35450.054.458979
GGGTCTA26700.054.4005172
GCCATAC35650.054.34514611
CCATACC36950.052.15571212
GCGCGGT66300.052.0529179
CCGAACT11250.051.90405367
CATACCA37350.051.5971513
CGCGGTG69550.049.96444310
GGCCATA39200.049.33641410
TGGCGCG70600.049.07691615
GCCGGTT11700.048.7732163
ATACCAC40050.048.4599614
TACCACC39300.048.25523415
CCTGGTT57750.047.9123270