FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298797
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG142370.43158157352513654No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94300.2858617853720614No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG64450.19537425309893272No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG39920.12101381200480053No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG35550.10776655853633917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT20300.039.28191870
ACCGTTA754.3173713E-836.5076641
CGCGGCA39750.036.2833732
GCGGCAC45800.033.92936733
TCGCCGG44400.033.45044748
CGGCACC46900.033.0076234
CCGTCGC45350.032.5542545
CCCGTCG45850.032.39892244
GACGTAT4800.031.96478344
ATGGCCG20500.030.91653869
GTTCCGT4100.030.8868161
AGGACCG8700.030.15837370
CCCCCGA53150.029.80988319
GCACCCC53550.029.53298836
TATCGGA2150.028.6072148
ACGTATC5250.028.58602745
CACCCCC55750.028.52025237
AGGAGGG78700.027.8478670
ACCCGCG56300.027.68265725
GGCACCC57150.027.28663435
Produced by FastQC (version 0.11.5)