FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264226
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG137010.4197319670880631No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91030.27887162224674394No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62820.19244991002461226No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG38940.1192932107029354No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36940.11316618395907636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA42050.036.9209232
TGGCCGT18400.035.84757670
GCGGCAC48050.035.2148533
TCGCCGG45650.034.90028448
CGGCACC48150.034.80363534
CCGTCGC46800.033.8375245
CCCGTCG48250.032.85588544
CGTACGT1155.0931703E-1132.76500319
CCCCCGA54400.031.60968819
GCACCCC54550.031.38735636
AGGACCG7850.030.19644470
GGCACCC56600.029.8680935
CACCCCC57100.029.7673837
AGGAGGG78900.029.39024470
ACCCGCG58900.029.27754625
CCCCCGT55150.028.88138442
ATGGCCG18250.028.53054469
CCCCGAC61150.027.96575720
AGTCCGC62500.027.424947
ACCGGTT2000.027.3676783